Monoclonal cGRP antibody
Mouse monoclonal antibody against a γ-carboxylated GRP epitope
Ref MA:C50 · 50 µg · €465
Snapshot
| Catalog number | MA:C50 |
|---|---|
| Target | Human γ-carboxylated Gla-rich protein (cGRP) |
| Host and clonality | Mouse, monoclonal |
| Reactivity | Human |
| Applications | Immunolocalization, immunofluorescence |
| Quantity and form | 50 µg, lyophilized |
| Purity | Affinity purified |
| Storage | -20 °C short term, -80 °C long term |
What it is for
GRP carries a high density of potential γ-carboxylation sites across its sequence, and the extent and distribution of carboxylation in native GRP remain largely undefined. No single antibody can resolve the overall carboxylation status of full-length GRP. Antibodies raised against individual carboxylation-sensitive epitopes are therefore the practical way to study epitope-specific GRP carboxylation in biological samples.
MA:C50 recognizes a γ-carboxylated epitope of human GRP and does not cross-react with the corresponding non-carboxylated peptide. Specificity was demonstrated with the matched synthetic peptides and by the distinct staining patterns obtained against MA:UC50 in tissue.
- • Comparative tissue localization using the matched MA:C50 and MA:UC50 pair
- • Assessment of GRP epitope carboxylation patterns in human tissues
Validated applications
| Application | Sample type | Recommended concentration |
|---|---|---|
| Immunolocalization | Human tissues and cells | 5 µg/ml |
| Immunofluorescence | Human tissues and cells | 5 µg/ml |
Optimize for your application.
Handling
Reconstitute with 50 µL sterile protease-free water for a final concentration of 1 µg/µL, by gentle rotation at 4 °C for at least 12 hours.
Shipped at room temperature, lyophilized.
After reconstitution store at -20 °C for up to two months. For long term storage use -80 °C.
Avoid repeated freeze-thaw cycles.
Key references
- Cavaco S et al. (2016). Gla-rich protein is involved in the cross-talk between calcification and inflammation in osteoarthritis. Cell Mol Life Sci 73(5):1051-1065. https://doi.org/10.1007/s00018-015-2033-9
The complete reference list is in the datasheet.
For research use only. Not intended for diagnostic, therapeutic or commercial use. Based on PCT patent application PCT/PT2009000046, owned by the University of Algarve and CCMAR, licensed to GenoGla Diagnostics.
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Mouse monoclonal against an uncarboxylated epitope of human GRP, the matched partner to MA:C50.
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Rabbit polyclonal detecting total GRP in all carboxylation forms, from fish to mammals.
Recombinant ucGRP protein
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Human non-carboxylated GRP expressed in E. coli, greater than 99% pure, for use as a standard or control.