Recombinant ucGRP protein
Human recombinant non-carboxylated GRP protein
Ref PTUC10 · 10 µg · €340
Snapshot
| Catalog number | PTUC10 |
|---|---|
| Source | Human recombinant, expressed in E. coli |
| Form | Lyophilized |
| Purity | Greater than 99% by HPLC and SDS-PAGE |
| Molecular weight | Approximately 14 kDa by SDS-PAGE |
| Quantity | 10 µg |
| Storage | -20 °C |
What it is for
Supplied as a non-carboxylated GRP reference protein. The recombinant protein comprises an N-terminal His6 tag, a V5 epitope, a TEV protease recognition site and the 74 amino acid mature human GRP sequence, 107 amino acids in total.
- • Standard or control in Western blot, immunoassays and functional studies
Handling
Lyophilized from the final RP-HPLC purification buffer, which contains residual trifluoroacetic acid.
Reconstitute with a minimum of 25 µL sterile protease-free water, giving a recommended maximum concentration of 0.2 µg/µL. Higher concentrations have not been validated and may reduce recovery.
Reconstitute by gentle pipetting followed by gentle rotation at 4 °C for at least 4 hours. Avoid vigorous mixing.
Shipped at room temperature. Store at -20 °C on arrival. Once reconstituted, aliquot if appropriate and avoid repeated freeze-thaw cycles.
Key references
- Viegas CS et al. (2014). Gla-rich protein is a potential new vitamin K target in cancer: evidences for a direct GRP-mineral interaction. BioMed Res Int 2014:340216. https://doi.org/10.1155/2014/340216
- Viegas CSB et al. (2022). Nanoencapsulation of Gla-rich protein as a novel approach to target inflammation. Int J Mol Sci 23(9):4813. https://doi.org/10.3390/ijms23094813
The complete reference list is in the datasheet.
For research use only. Not intended for diagnostic, therapeutic or commercial use. Based on PCT patent application PCT/PT2009000046, owned by the University of Algarve and CCMAR, licensed to GenoGla Diagnostics.
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