Total GRP analysis service
We measure total GRP in your samples
The only GRP ELISA validated in peer-reviewed articles (Biochem Med 2026, ATVB 2018), with well-defined capture and detection antibodies and supported by peer-reviewed analytical validation and clinical application studies.
Its specificity, analytical performance and molecular target are fully documented, providing confidence and transparency in tGRP quantification across multiple biological matrices and clinical research settings.
Snapshot
| Assay format | Sandwich ELISA |
|---|---|
| Target | Human total GRP |
| Validated matrices | Serum, peritoneal dialysate, cell culture supernatant, RIPA protein extracts |
| Dynamic range | 60 to 1600 pg/mL |
| Assay sample volume | 100 µL |
| Intra-assay precision | 1.4% to 3.2% CV |
| Inter-assay precision | 2.5% to 9.4% CV |
What it is for
Quantitative determination of human total Gla-rich protein using a validated sandwich ELISA, run in our laboratory on samples you send us. The assay has been analytically validated across several biological matrices, supporting basic, translational and clinical research. Other sample types can be evaluated on request.
Every analytical run includes internal quality controls checked against predefined acceptance criteria.
Sample requirements
- • Minimum 150 µL per sample for single-well analysis. At least 250 µL for duplicate measurement, which we recommend. Different sample volumes may be required depending on sample concentration or if repeat analysis is requested.
- • Store at -80 °C whenever possible and ship on dry ice.
- • Avoid repeated freeze-thaw cycles.
- • Label samples with anonymized or coded identifiers only. No names or personal identifiers. Include a completed sample submission form.
- • All analyses are performed blinded to sample identity and study group.
- • If your collection or storage conditions differ from the above, contact us before shipping and we will advise on suitability.
What you receive
| Result | Total GRP concentration in pg/mL for each sample |
|---|---|
| Format | Excel spreadsheet and PDF report |
| Quality control | Standard curve, acceptance criteria and internal controls |
| Replicates | Duplicate measurements unless you request otherwise |
| Interpretation | Summary of assay performance and comments on samples outside the validated range |
Raw ELISA data is available on request. Statistical analysis, graphical presentation and scientific consultation on study design and data interpretation are available as options.
Key references
- Viegas CSB et al. (2018). Chronic kidney disease circulating calciprotein particles and extracellular vesicles promote vascular calcification: a role for GRP. Arterioscler Thromb Vasc Biol 38(3):575-587. https://doi.org/10.1161/ATVBAHA.117.310578
- Silva AP et al. (2020). Gla-rich protein (GRP) as an early and novel marker of vascular calcification and kidney dysfunction in diabetic patients with CKD: a pilot cross-sectional study. J Clin Med 9(3):635. https://doi.org/10.3390/jcm9030635
- Silva AP et al. (2022). Gla-rich protein, magnesium and phosphate associate with mitral and aortic valves calcification in diabetic patients with moderate CKD. Diagnostics 12(2):496. https://doi.org/10.3390/diagnostics12020496
- Marreiros C et al. (2024). Gla-rich protein is associated with vascular calcification, inflammation, and mineral markers in peritoneal dialysis patients. J Clin Med 13(23):7429. https://doi.org/10.3390/jcm13237429
- Marreiros C et al. (2026). Factors associated with Gla-rich protein serum concentrations in healthy adults. Biochem Med (Zagreb) 36(2):020709. https://doi.org/10.11613/BM.2026.020709
The complete reference list is in the datasheet.
For research use only. Not intended for diagnostic, therapeutic or commercial use. Based on PCT patent application PCT/PT2009000046, owned by the University of Algarve and CCMAR, licensed to GenoGla Diagnostics.
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