C-Term GRP polyclonal antibody
Rabbit polyclonal antibody against total GRP, all carboxylation forms
Ref PA:T50 · 50 µg · €360
Snapshot
| Catalog number | PA:T50 |
|---|---|
| Target | Total GRP, all forms |
| Host and clonality | Rabbit, polyclonal |
| Reactivity | Multi-species, fish to mammals |
| Applications | Western blot, immunolocalization, immunofluorescence, flow cytometry, immunogold electron microscopy |
| Quantity and form | 50 µg, lyophilized |
| Purity | Affinity purified |
| Storage | -20 °C short term, -80 °C long term |
What it is for
GRP is a vitamin K-dependent protein with 15 putative γ-carboxyglutamic acid residues in humans. It inhibits calcification in the cardiovascular and articular systems and acts as an anti-inflammatory agent in several cell types. It is present in serum and accumulates at sites of pathological calcification.
PA:T50 recognizes GRP independently of carboxylation state and reacts across species from fish to mammals. It shows no significant cross-reactivity with other Gla-containing proteins such as matrix Gla protein and osteocalcin.
- • Detecting, localizing and characterizing total GRP across tissues, cells, nanoparticles, extracellular vesicles and protein extracts
Confirmed species reactivity
| Species | Validated by |
|---|---|
| Human | Western blot, vascular system; immunolocalization, several tissues |
| Rat | Western blot, skin; immunolocalization, several tissues |
| Mouse | Western blot, skin |
| Pig | Western blot, skin and elastic cartilage |
| Rabbit | Western blot, skin |
| Sturgeon | Western blot, purified GRP protein |
Validated applications
| Application | Recommended concentration |
|---|---|
| Western blot | 5 µg/ml |
| Immunolocalization | 5 µg/ml |
Optimize for your application.
Handling
Reconstitute with 50 µL sterile protease-free water, by gentle rotation at 4 °C for at least 12 hours.
Shipped at room temperature, lyophilized.
After reconstitution store at -20 °C for up to two months. For long term storage use -80 °C.
Avoid repeated freeze-thaw cycles.
Key references
- Viegas CS et al. (2009). Gla-rich protein is a novel vitamin K-dependent protein present in serum and accumulated at sites of pathological calcifications. Am J Pathol 175(6):2288-2298. https://doi.org/10.2353/ajpath.2009.090474
- Viegas CSB et al. (2018). Chronic kidney disease circulating calciprotein particles and extracellular vesicles promote vascular calcification: a role for GRP. Arterioscler Thromb Vasc Biol 38(3):575-587. https://doi.org/10.1161/ATVBAHA.117.310578
- Viegas C et al. (2026). Bioengineered baculovirus-derived extracellular vesicles loaded with γ-carboxylated Gla-rich protein: dual modulation of inflammation and vascular calcification. Biomater Adv 184:214833. https://doi.org/10.1016/j.bioadv.2026.214833
The complete reference list is in the datasheet.
For research use only. Not intended for diagnostic, therapeutic or commercial use. Based on PCT patent application PCT/PT2009000046, owned by the University of Algarve and CCMAR, licensed to GenoGla Diagnostics.
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