Monoclonal ucGRP antibody
Mouse monoclonal antibody against an uncarboxylated GRP epitope
Ref MA:UC50 · 50 µg · €465
Snapshot
| Catalog number | MA:UC50 |
|---|---|
| Target | Human undercarboxylated Gla-rich protein (ucGRP) |
| Host and clonality | Mouse, monoclonal |
| Reactivity | Human |
| Applications | Immunolocalization, immunofluorescence |
| Quantity and form | 50 µg, lyophilized |
| Purity | Affinity purified |
| Storage | -20 °C short term, -80 °C long term |
What it is for
GRP carries a high density of potential γ-carboxylation sites across its sequence, and the extent and distribution of carboxylation in native GRP remain largely undefined. No single antibody can resolve the overall carboxylation status of full-length GRP. Antibodies raised against individual carboxylation-sensitive epitopes are therefore the practical way to study epitope-specific GRP carboxylation in biological samples.
MA:UC50 recognizes an uncarboxylated epitope of human GRP and does not cross-react with the corresponding γ-carboxylated peptide. Specificity was demonstrated with the matched synthetic peptides and by the distinct staining patterns obtained against MA:C50 in tissue.
- • Comparative tissue localization using the matched MA:UC50 and MA:C50 pair
- • Assessment of GRP epitope carboxylation patterns in human tissues
Validated applications
| Application | Sample type | Recommended concentration |
|---|---|---|
| Immunolocalization | Human tissues and cells | 5 µg/ml |
| Immunofluorescence | Human tissues and cells | 5 µg/ml |
Optimize for your application.
Handling
Reconstitute with 50 µL sterile protease-free water for a final concentration of 1 µg/µL, by gentle rotation at 4 °C for at least 12 hours.
Shipped at room temperature, lyophilized.
After reconstitution store at -20 °C for up to two months. For long term storage use -80 °C.
Avoid repeated freeze-thaw cycles.
Key references
- Viegas CS et al. (2014). Gla-rich protein is a potential new vitamin K target in cancer: evidences for a direct GRP-mineral interaction. BioMed Res Int 2014:340216. https://doi.org/10.1155/2014/340216
- Cavaco S et al. (2016). Gla-rich protein is involved in the cross-talk between calcification and inflammation in osteoarthritis. Cell Mol Life Sci 73(5):1051-1065. https://doi.org/10.1007/s00018-015-2033-9
- Viegas CS et al. (2015). Gla-rich protein acts as a calcification inhibitor in the human cardiovascular system. Arterioscler Thromb Vasc Biol 35(2):399-408. https://doi.org/10.1161/ATVBAHA.114.304823
The complete reference list is in the datasheet.
For research use only. Not intended for diagnostic, therapeutic or commercial use. Based on PCT patent application PCT/PT2009000046, owned by the University of Algarve and CCMAR, licensed to GenoGla Diagnostics.
Related products
Total GRP analysis service
Quantitative total GRP measurement by validated sandwich ELISA, run in our laboratory on your samples.
Monoclonal cGRP antibody
Ref MA:C50 · 50 µg
Mouse monoclonal against a γ-carboxylated epitope of human GRP, for tissue and cell localization.
C-Term GRP polyclonal antibody
Ref PA:T50 · 50 µg
Rabbit polyclonal detecting total GRP in all carboxylation forms, from fish to mammals.
Recombinant ucGRP protein
Ref PTUC10 · 10 µg
Human non-carboxylated GRP expressed in E. coli, greater than 99% pure, for use as a standard or control.